Join our email list


Sign up here to be kept updated via email.

JOIN OUR EMAIL LIST

We are extremely sad to learn of Professor Mark Wainberg’s death. Mark was a great colleague and active Trustee. His contribution to HIV research, advocacy and his mentorship for so many young researchers across the globe has made genuine differences to so many lives. We will miss his tremendous support, input and encouragement.
Suzaan Marais
Country South Africa
Job Title Senior Research Officer
Date of Scholarship May-July 2011
Host Country London, UK
Scholarship Investigating the presence of small regulatory RNAs (sRNAs) in cerebrospinal fluid (CSF) samples of patients with tuberculous meningitis (TBM)

 

During my visit to the National Institute for Mycobacterial Research (NIMR) in London, I acquired skills to perform Mycobacterium tuberculosis (M.tb) sRNA extraction, amplification and PCR from cerebrospinal fluid (CSF) samples of patients with tuberculous meningitis. After RNA isolation, human RNA was depleted from samples employing a capture hybridization method. This was followed by an in vitro transcription-based RNA amplification procedure. Quantitive RT-PCR was subsequently performed using commercially available and/or in-house designed probes and primers for M.tb RNA. Concentrations of ribosomal RNAs, sRNAs as well as messenger RNAs were measured. Detection of sRNAs at levels comparable to ribosomal RNA confirms their potential as sensitive biomarkers and provides the first evidence of the presence of M.tb sRNAs in bacilli from humans with tuberculosis.

With my gained knowledge of RNA analysis techniques, myself and other scientists at the University of Cape Town and the NIMR continue to explore the presence and significance of M.tb sRNAs in patients with tuberculosis. The results of our experiments have also contributed substantially to the success of my Wellcome trust Research Training Fellowship application and will contribute largely to my PhD.

I would like to thank the HIV Research Trust sincerely for its role in advancing my research career.



Back

2017 Timeline

  • Application for 2018 scholarships opens 1 September 2017
  • Online application closes 20 October 2017
  • 2018 scholarships announced late December 2017

JOIN OUR EMAIL LIST

HOW TO APPLY


If you are interested in applying for a scholarship find out more below:

HOW TO APPLY

AM I ELIGIBLE TO APPLY?


The scholarships are for health care professionals in early/mid-career who are located in resource-limited settings from low-income and lower-middle-income countries working in the field of HIV infection. Read more to see if you are eligible.